Imaging and Spectroscopic Analysis of Living Cells: Optical and Spectroscopic Techniques

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Volume info:
Series: Methods in Enzymology 504
Author: P. Michael conn (Eds.)
Year: 2012
Edition: 1
Publisher: Academic Press
Pages: 2-506
PagesInFile: 529
Language: English
Library issue:

DPI: 0
Searchable: 1


ISBN: 9780123918574
ISSN: 0076-6879
Googlebook id:
Openlibrary id:

CRC32: E8F2A9F2
SHA256: 6651555BE4328DE37C662026DB1704661B6A8FED409B94ECF39E2320636C4D6C
MD5: 336926a0e3acc747e8ef4dc248f62057


Series Page
Page ii

Copyright Page
Page iv

Pages xi-xvi

Page xvii
P. Michael Conn

Volume in series
Pages xix-xlviii

Chapter one - Laser-Induced Radiation Microbeam Technology and Simultaneous Real-Time Fluorescence Imaging in Live Cells
Pages 3-28
Stanley W. Botchway, Pamela Reynolds, Anthony W. Parker, Peter O'Neill

Chapter Two - A Cell Biologist's Guide to High Resolution Imaging
Pages 29-55
Graeme Ball, Richard M. Parton, Russell S. Hamilton, Ilan Davis

Chapter Three - Applications of Fluorescence Lifetime Spectroscopy and Imaging to Lipid Domains In Vivo
Pages 57-81
André E.P. Bastos, Silvia Scolari, Martin Stöckl, Rodrigo F.M. de Almeida

Chapter four - Detecting and Tracking Nonfluorescent Nanoparticle Probes in Live Cells
Pages 83-108
Gufeng Wang, Ning Fang

Chapter five - Fluorescence Lifetime Microscopy of Tumor Cell Invasion, Drug Delivery, and Cytotoxicity
Pages 109-125
Gert-Jan Bakker, Volker Andresen, Robert M. Hoffman, Peter Friedl

Chapter six - Measuring Membrane Protein Dynamics in Neurons Using Fluorescence Recovery after Photobleach
Pages 127-146
Inmaculada M. GonzГЎlez-GonzГЎlez, FrГ©dГ©ric Jaskolski, Yves Goldberg, Michael C. Ashby, Jeremy M. Henley

Chapter seven - Fluorescent Speckle Microscopy in Cultured Cells
Pages 147-161
Marin Barisic, AntГіnio J. Pereira, Helder Maiato

Chapter Eight - Green-to-Red Photoconvertible mEosFP-Aided Live Imaging in Plants
Pages 163-181
Jaideep Mathur, Sarah Griffiths, Kiah Barton, Martin H. Schattat

Chapter nine - Methods for Cell and Particle Tracking
Pages 183-200
Erik Meijering, Oleh Dzyubachyk, Ihor Smal

Chapter ten - Correlative Light-Electron Microscopy: A Potent Tool for the Imaging of Rare or Unique Cellular and Tissue Events and Structures
Pages 201-219
Alexander A. Mironov, Galina V. Beznoussenko

Chapter eleven - Optical Techniques for Imaging Membrane Domains in Live Cells (Live-Cell Palm of Protein Clustering)
Pages 221-235
Dylan M. Owen, David Williamson, Astrid Magenau, JГ©rГ©mie Rossy, Katharina Gaus

Chapter twelve - Single Live Cell Topography and Activity Imaging with the Shear-Force-Based Constant-Distance Scanning Electrochemical Microscope
Pages 237-254
Albert Schulte, Michaela Nebel, Wolfgang Schuhmann

Chapter thirteen - Visualization of TGN-Endosome Trafficking in Mammalian and Drosophila Cells
Pages 255-271
Satoshi Kametaka, Satoshi Waguri

Chapter fourteen - Live Cell Imaging with Chemical Specificity Using Dual Frequency CARS Microscopy
Pages 273-291
Iestyn Pope, Wolfgang Langbein, Paola Borri, Peter Watson

Chapter fifteen - Imaging Intracellular Protein Dynamics by Spinning Disk Confocal Microscopy
Pages 293-313
Samantha Stehbens, Hayley Pemble, Lyndsay Murrow, Torsten Wittmann

Chapter sixteen - Visualizing Dynamic Activities of Signaling Enzymes Using Genetically Encodable Fret-Based Biosensors: From Designs to Applications
Pages 317-340
Xin Zhou, Katie J. Herbst-Robinson, Jin Zhang

Chapter seventeen - Live-Cell Imaging of Aquaporin-4 Supramolecular Assembly and Diffusion
Pages 341-354
A.S. Verkman, Andrea Rossi, Jonathan M. Crane

Chapter eighteen - Coiled-Coil Tag–Probe Labeling Methods for Live-Cell Imaging of Membrane Receptors
Pages 355-370
Yoshiaki Yano, Kenichi Kawano, Kaoru Omae, Katsumi Matsuzaki

Chapter nineteen - Monitoring Protein Interactions in Living Cells with Fluorescence Lifetime Imaging Microscopy
Pages 371-391
Yuansheng Sun, Nicole M. Hays, Ammasi Periasamy, Michael W. Davidson, Richard N. Day

Chapter twenty - Open Source Tools for Fluorescent Imaging
Pages 393-417
Nicholas A. Hamilton

Chapter twenty-one - Nanoparticle PEBBLE Sensors in Live Cells
Pages 419-470
Yong-Eun Koo Lee, Raoul Kopelman

Author Index
Pages 471-494

Subject Index
Pages 495-506


This volume of Methods in Enzymology is the first of 3 parts looking at current methodology for the imaging and spectroscopic analysis of live cells. The chapters provide hints and tricks not available in primary research publications. It is an invaluable resource for academics, researchers and students alike.

  • Expert authors who are leaders in the field
  • Extensively referenced and useful figures and tables
  • Provides hints and tricks to facilitate reproduction of methods
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